PATHOLOGIC CHANGES IN EXTRAHEPATIC ORGANS AND AGGLUTININ RESPONSE TO SALMONELLA GALLINARUM INFECTION IN JAPANESE QUAIL FED FUSARIUM VERTICILLIOIDES CULTURE MATERIAL CONTAINING KNOWN LEVELS OF FUMONISIN B1

2007 ◽  
Vol 2 (3) ◽  
pp. e22-e22
Author(s):  
S. DESHMUKH ◽  
R. K. ASRANI ◽  
D. R. LEDOUX ◽  
G. E. ROTTINGHAUS ◽  
A. J. BERMUDEZ ◽  
...  
Keyword(s):  
Japanese Quail ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Salmonella Gallinarum ◽  
Culture Material

Fumonisin B1-4-producing capacity of Hungarian Fusarium verticillioides isolates

2010 ◽  
Vol 3 (1) ◽  
pp. 67-76 ◽  
Author(s):  
Á. Szécsi ◽  
A. Szekeres ◽  
T. Bartók ◽  
G. Oros ◽  
M. Bartók ◽  
...  
Keyword(s):  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Cultural Characteristics ◽  
The Third ◽  
Rice Grains ◽  
Rp Hplc ◽  
Culture Material ◽  
Analytical Results ◽  
Different Sources

The fumonisin B1-4 (FB1-4) toxin-producing capacities of 60 strains of Fusarium verticillioides isolated in the main maize-cultivating areas of Hungary were screened on rice grains in vitro. The amounts of FB1, FB2, FB3 and FB4 in the extracts of the culture material were determined by RP-HPLC/ESI-IT MS without any sample clean-up. All F. verticillioides strains produced all analogues (FB1-4) of the fumonisin B series. The strains did not differ significantly in their cultural characteristics, though the analytical results allowed the distinction of three idiosyncratic FB1-4-producing chemotypes with characteristic proportions of the fumonisin B analogues. The dominant chemotype produced them in the sequence FB1 > FB2 > FB3 > FB4, with large amounts of FB1 and FB2. A second chemotype produced a higher amount of FB3 than of FB2, while the third chemotype produced large amounts of FB2 and FB4. The proportion of FB1 in the extracts was inversely correlated with the total FB yield. No differences in FB1-4 producing capacity were observed between strains isolated from various locations or different sources.

Toxicity of Fumonisin from Fusarium verticillioides Culture Material and Moniliformin from Fusarium fujikuroi Culture Material When Fed Singly and in Combination to Growing Barrows

2002 ◽  
Vol 65 (2) ◽  
pp. 373-377 ◽  
Author(s):  
ROGER B. HARVEY ◽  
THOMAS S. EDRINGTON ◽  
LEON F. KUBENA ◽  
GEORGE E. ROTTINGHAUS ◽  
JAMES R. TURK ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Diet Group ◽  
Fusarium Fujikuroi ◽  
Culture Material ◽  
Feed Control ◽  
Serum Biochemical

The effects of fumonisin B1 (FB1) from Fusarium verticillioides culture material and moniliformin from Fusarum fujikuroi culture material on growing barrows were evaluated. Four groups of six barrows (three replicates of two each; mean body weight, 11.1 kg) were fed diets containing 0 mg of FB1 and 0 mg of moniliformin per kg of feed (control), 100 mg of FB1 per kg of feed, 100 mg of moniliformin per kg of feed, and 100 mg of FB1 plus 100 mg of moniliformin per kg of feed. Barrows were fed these diets for 28 days. Body weight gain, feed efficiency, serum biochemical analytes, and hematological values were adversely affected by the FB1 and the FB1-plus-moniliformin diets. The moniliformin diet decreased body weight gain. Two barrows in the moniliformin diet group died, and two barrows in the FB1-plus-moniliformin diet group died. All deaths occurred during the first 6 days of the study. Mild to moderate lesions were observed microscopically in heart and lung tissues of the groups fed moniliformin and FB1 plus moniliformin and in liver tissues of the groups fed FB1 and FB1 plus moniliformin. Except for the acute mortality associated with the two diets containing moniliformin, clinical disease induced by the combined feeding of these two mycotoxins appears to be additive or less than additive and due primarily to the toxic expression of FB1.

Mortality in broiler chicks on feed amended withFusarium proliferatum culture material or with purified fumonisin B1 and moniliformin

1993 ◽  
Vol 123 (3) ◽  
pp. 171-184 ◽  
Author(s):  
T. Javed ◽  
G. A. Bennett ◽  
J. L. Richard ◽  
M. A. Dombrink-Kurtzman ◽  
L. M. C�t� ◽  
...  
Keyword(s):  
Fumonisin B1 ◽  
Broiler Chicks ◽  
Culture Material

scholarly journals Fusarium verticillioides FvPex8 is a key component of peroxisomal docking/translocation module that serves important roles in fumonisin biosynthesis but not in virulence

2020 ◽  
Author(s):  
Wenying Yu ◽  
Mei Lin ◽  
Minghui Peng ◽  
Huijuan Yan ◽  
Jie Zhou ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Gene Expression Profiles ◽  
Deletion Mutants ◽  
Altered Expression ◽  
The Core ◽  
Metabolic Functions ◽  
Peroxisome Membrane

AbstractPeroxisomes are ubiquitous organelles in eukaryotic cells that fulfill various important metabolic functions. In this study, we investigated the role of Docking/Translocation Module (DTM) peroxins, mainly FvPex8, FvPex13, FvPex14, and FvPex33, in Fusarium verticillioides virulence and fumonisin B1 (FB1) biosynthesis. Protein interaction experiments suggested that FvPex13 serves as the core subunit of F. verticillioides DTM. When we generated gene deletion mutants (ΔFvpex8, ΔFvpex13, ΔFvpex14, ΔFvpex33, ΔFvpex33/14) and examined whether the expression of other peroxin genes were affected in the DTM mutants, ΔFvpex8 strain showed most drastic changes to PEX gene expression profiles. Deletion mutants exhibited disparity in carbon source utilization and defect in cell wall integrity when stress agents were applied. Under nutrient starvation, mutants also showed higher levels of lipid droplet accumulation. Notably, ΔFvpex8 mutant showed significant FB1 reduction and altered expression of FUM1 and FUM19 genes. However, FvPex13 was primarily responsible for virulence, while ΔFvpex33/14 double mutant also showed virulence defect. In summary, our study suggests that FvPex13 is the core component of DTM, regulating peroxisome membrane biogenesis as well as PTS1- and PTS2-mediated transmembrane cargo transportation. Importantly, we predict FvPex8 as a key component in DTM that affects peroxisome function in FB1 biosynthesis in F. verticillioides.

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